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Published on: February 15, 2019
Composition of "gold juice" using an ancient method based on intestinal microecology
Qiuwei Li1, Liying Guo1, Li Wang1
1Tianjin Second People's Hospital, Tianjin, China.
Insights
Gold juice, a traditional Chinese medicine, contains unique bacteria that synthesize carbohydrates and amino acids. This finding helps understand its effectiveness for fecal microbiota transplantation.
Area of Science:
- Microbiology
- Traditional Chinese Medicine
- Gastroenterology
Background:
- Gold juice is a traditional Chinese medicine used in fecal microbiota transplantation.
- Understanding its microbial composition is crucial for optimizing its therapeutic potential.
Purpose of the Study:
- To identify the specific bacterial components within gold juice.
- To compare the microbial profile of gold juice with its filtrate.
Main Methods:
- Fecal samples from healthy children were used to prepare gold juice.
- 16S rDNA sequencing and QIIME software were employed for microbial analysis.
- Comparisons were made between gold juice, diluent, filtrate, and loess samples.
Main Results:
- Gold juice processing significantly altered its microflora.
- Distinct microbial differences were observed between gold juice and filtrate.
- Gold juice primarily contained carbohydrate- and amino acid-synthesizing microbes, unlike the filtrate.
Conclusions:
- This study identified key bacterial components in gold juice.
- The findings provide evidence for effective bacterial components in gold juice.
- Results may inform clinical applications of gold juice in fecal microbiota transplantation.
Objective:
To identify potentially effective bacterial components of gold juice, a traditional Chinese medicine treatment used for fecal microbiota transplantation.
Methods:
Fecal samples were collected from five healthy children (two boys and three girls; mean age, 7.52 ± 2.31 years). The children had no history of antibiotic use or intestinal microecological preparation in the preceding 3 months. Fresh fecal samples were collected from children to prepare gold juice in mid-to-late November, in accordance with traditional Chinese medicine methods, then used within 7 days. Finally, 16S rDNA sequence analysis was used to identify potentially effective bacterial components of gold juice. QIIME software was used for comparisons of microbial species among gold juice, diluent, filtrate, and loess samples.
Results:
Microflora of gold juice exhibited considerable changes following "ancient method" processing. Microbial components significantly differed between gold juice and filtrate samples. The gold juice analyzed in our study consisted of microbes that synthesize carbohydrates and amino acids by degrading substances, whereas the filtrate contained probiotic flora, Bacteroides, and Prevotella 9.
Conclusions:
This study of microbial components in gold juice and filtrate provided evidence regarding effective bacterial components in gold juice, which may aid in clinical decisions concerning fecal microbiota transplantation.
