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Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

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Related Experiment Video

Updated: Dec 7, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

TRIP - T cell receptor/immunoglobulin profiler.

Maria Th Kotouza¹, Katerina Gemenetzi², Chrysi Galigalidou²

¹Department of Electrical and Computer Engineering, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.

BMC Bioinformatics

|September 30, 2020

Summary

This summary is machine-generated.

The T cell Receptor/Immunoglobulin Profiler (TRIP) tool analyzes antigen receptor sequences from next-generation sequencing data. It accurately processes gene rearrangements and somatic hypermutation, offering tailored data analysis and visualization for researchers.

Keywords:

Antigen receptor R shiny Software pipeline

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Last Updated: Dec 7, 2025

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

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Published on: January 12, 2021

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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Area of Science:

Immunoinformatics
Computational Biology
Genomics

Background:

High-throughput immunoprofiling via next-generation sequencing (NGS) presents computational challenges due to extreme antigen receptor diversity.
The T cell Receptor/Immunoglobulin Profiler (TRIP) tool processes IMGT/HighV-Quest output for in-depth analysis of antigen receptor gene rearrangements.
TRIP provides detailed insights into V(D)J gene usage, CDR3 composition, clonality, and somatic hypermutation in T cell receptors (TR) and B cell receptor immunoglobulins (BcR IG).

Purpose of the Study:

To introduce TRIP, a web application for analyzing antigen receptor gene sequence data.
To provide a comprehensive framework for data wrangling, cleaning, analysis, and visualization of immunoprofiling data.
To offer a customizable pipeline for researchers studying antigen receptor diversity.

Main Methods:

TRIP is a web application implemented in R Shiny.
It processes output files from the IMGT/HighV-Quest tool.
The tool utilizes a modular approach for analyzing V(D)J gene rearrangements and somatic hypermutation.

Main Results:

TRIP demonstrated linear response time, processing 1 million sequences in approximately 6 hours for the BcR IG pipeline.
Reproducibility tests showed no significant differences compared to a previous pipeline on the Galaxy platform.
A stricter preselection process in TRIP filtered out approximately 0.1% more rearrangements without impacting final results.

Conclusions:

TRIP is an accurate software framework for analyzing antigen receptor gene sequence data.
It offers essential functions for data manipulation, analysis, and visualization.
TRIP enables users to construct personalized analytical pipelines and is publicly accessible.