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AtHDA15 binds directly to COP1 positively regulating photomorphogenesis.

Malona V Alinsug1, Amandine Radziejwoski2, Custer C Deocaris3

  • 1Institute of Plant Biology, College of Life Sciences, National Taiwan University, Taipei, Taiwan; Center for Food & Bio Convergence, College of Agriculture & Life Sciences, Seoul National University, South Korea; Science Department, College of Natural Sciences & Mathematics, Mindanao State University-General Santos City, Philippines.

Biochemical and Biophysical Research Communications
|September 30, 2020
PubMed
Summary

Histone deacetylase HDA15 positively regulates photomorphogenesis by directly interacting with COP1, a key repressor of light signaling in plants. This interaction modulates COP1 activity, impacting seedling development and gene expression in response to light.

Keywords:
COP1HDA15HY5Histone deacetylasePhytochrome signaling

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Histone acetylation/deacetylation regulates light-induced gene expression in plants.
  • The precise role of histone-modifying enzymes in light signaling pathways is not fully understood.

Purpose of the Study:

  • To investigate the function of Histone Deacetylase 15 (HDA15) in photomorphogenesis.
  • To elucidate the molecular mechanism by which HDA15 interacts with the light signaling network.

Main Methods:

  • T-DNA knock-out and RNAi lines were used to study HDA15 function.
  • In vivo and in vitro binding assays were performed to confirm HDA15-COP1 interaction.
  • Analysis of hypocotyl length and protein levels (HY5, PIF3) under different light conditions.

Main Results:

  • HDA15 positively regulates photomorphogenesis.
  • hda15 mutants show light hyposensitivity, longer hypocotyls in dark, and reduced HY5/PIF3 levels.
  • HDA15 directly binds to COP1 in the nucleus, modulating its repressive activity.
  • Overexpression of HDA15 leads to increased HY5 and shorter hypocotyls.

Conclusions:

  • HDA15 directly interacts with COP1, a master regulator of photomorphogenesis.
  • This interaction is crucial for modulating COP1's repressive functions in light and dark.
  • HDA15's role extends beyond histone modification, highlighting its function in post-translational regulation within light signaling.