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Nanoceria: Metabolic interactions and delivery through PLGA-encapsulation
Apoorva Mehta1, Bradley Scammon1, Kevin Shrake1
1Department of Bioengineering, Clemson University, 301 Rhodes Hall, Clemson, SC 29634, USA.
Materials Science & Engineering. C, Materials for Biological Applications
|September 30, 2020
Summary
Cerium oxide nanoparticles (nanoceria) and superoxide dismutase (SOD) were encapsulated in PLGA for combined antioxidant therapy. This dual delivery system effectively reduces oxidative stress in cells, showing promise for biomedical applications.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Biochemistry
Background:
- Cerium oxide nanoparticles (nanoceria) possess recyclable antioxidant properties, mimicking enzymes like superoxide dismutase (SOD) in scavenging reactive oxygen species (ROS).
- Nanoceria's ability to protect cells from oxidative stress has led to investigations into combination therapies with metabolic enzymes.
- A complementary relationship observed between SOD and nanoceria motivated the development of a simultaneous delivery system.
Purpose of the Study:
- To develop and evaluate a method for the simultaneous delivery of SOD and nanoceria.
- To assess the suitability of poly(lactic-co-glycolic acid) (PLGA) as an encapsulating material for both SOD and nanoceria.
- To verify the in vitro efficacy of the combined PLGA-nanoceria-SOD particles in reducing oxidative stress.
Main Methods:
- Encapsulation of nanoceria and SOD within PLGA nanoparticles.
- Conducting cellular uptake and cytotoxicity assays.
- Verifying antioxidative properties using hydrogen peroxide (H2O2) challenge and fluorescent imaging of oxidative stress markers.
Main Results:
- PLGA demonstrated biocompatibility and tunable degradation, suitable for co-encapsulating SOD and nanoceria.
- Cellular uptake studies confirmed the internalization of the PLGA-nanoceria-SOD particles.
- In vitro experiments showed that the combined particles effectively reduced oxidative stress induced by H2O2.
Conclusions:
- PLGA serves as an effective carrier for the simultaneous delivery of nanoceria and SOD.
- The combination of nanoceria and SOD delivered via PLGA shows significant potential for reducing cellular oxidative stress in vitro.
- This approach offers a promising strategy for combination therapy in biomedical applications targeting oxidative damage.
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