Effects of resveratrol/ethanol pretreatment on dentin bonding durability

To determine the effects of resveratrol/ethanol solution on the durability of resin-dentin bonding interfaces. Sixty-four non-caries third molars were randomly divided into four groups (n = 16) after sectioning, and then pretreated with one of the following concentrations of resveratrol/ethanol solutions: 0 (control group), 1, 10 and 20 mg/mL, followed by a universal adhesive and resin composites. All microtensile samples were divided into three subgroups: immediate group, collagenase ageing group and thermocycled group. The microtensile bond strength (MTBS), failure modes, interfacial nanoleakage and in situ zymography were measured, whereas the inhibitory effects of pretreated dentin slices on S. mutans biofilms were determined by confocal laser scanning microscopy and MTT assay. The results indicated that bonding strength was not only influenced by pretreatment factors (P < 0.05) but also ageing factors (P < 0.05). Regardless of collagenase ageing or thermocycling, the 10 mg/mL resveratrol/ethanol pretreatment group presented significantly higher (P < 0.05) MTBS and lower (P < 0.05) expression of nanoleakage than the control group, showed better inhibitory effect of matrix metalloproteinases and S. mutans activity with acceptable cytotoxicity. Meanwhile, cohesive failure in dentin decreased gradually with increasing resveratrol concentration. Therefore, the resveratrol/ethanol solution had the potential to serve as a versatile dentin primer, which can effectively improve dentin bonding durability and prevent secondary caries.