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Related Experiment Video

Updated: Dec 7, 2025

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Published on: February 22, 2015

Myelin replacement triggered by single-cell demyelination in mouse cortex.

Nicolas Snaidero^1,2,3,4, Martina Schifferer^5,6, Aleksandra Mezydlo^7,8

¹Institute of Neuronal Cell Biology, Technische Universität München, 80802, Munich, Germany. nicolas.snaidero@tum.de.

Nature Communications

|September 30, 2020

Summary

Loss of a single oligodendrocyte in mouse cortex triggers cell replacement and remyelination. Continuous myelin patterns show resilience, while patchy myelination is not reestablished, revealing myelin plasticity insights.

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Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

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Last Updated: Dec 7, 2025

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Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

Published on: March 26, 2015

Area of Science:

Neuroscience
Cell Biology
Neurodegeneration and Remyelination Research

Background:

Myelin, traditionally viewed as a static axonal insulator, is increasingly recognized for its dynamic role in neural circuit plasticity.
Precise regulation of oligodendrocyte populations and myelination patterns is crucial for maintaining nervous system function.
Understanding the regenerative capacity of myelin following injury is vital for developing therapeutic strategies.

Purpose of the Study:

To investigate the cellular and molecular responses to subtle demyelination at the single oligodendrocyte level.
To determine the capacity for myelin pattern reestablishment after targeted oligodendrocyte loss in the mouse cortex.
To elucidate the mechanisms governing remyelination of continuously versus partially myelinated axons.

Main Methods:

Development of a precise laser ablation technique to eliminate individual oligodendrocytes in vivo.
Longitudinal in vivo imaging to track cellular responses and myelination dynamics post-ablation.
Correlated ultrastructural reconstructions to provide high-resolution anatomical detail of myelin repair.

Main Results:

Demyelination, even from the loss of a single oligodendrocyte, initiates robust oligodendrocyte replacement and remyelination.
Remyelination successfully reestablishes original myelin patterns on continuously myelinated axons with high fidelity.
Previously unmyelinated axons develop new, patchy internodes, while internodes on partially myelinated axons are not typically reestablished.

Conclusions:

Mammalian cortical myelin exhibits remarkable homeostatic resilience, particularly in continuously myelinated segments.
The cues guiding patchy myelination appear not to be preserved across repeated de- and remyelination cycles.
These findings highlight distinct mechanisms for myelin repair based on pre-existing myelination patterns, offering insights into myelin plasticity.