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Ganoderma lucidum put forth anti-tumor activity against PC-3 prostate cancer cells via inhibition of Jak-1/STAT-3

Xiaoming Wang1, Bo Wang1, Liquan Zhou1

  • 1Department of Urology, The Second Affiliated Hospital of Guangxi Medical University, Nanning City, Guangxi Province 530007, China.

Saudi Journal of Biological Sciences
|September 30, 2020
PubMed
Abstract

Insights

Ganoderma lucidum exhibits anticancer effects against prostate cancer cells by inhibiting the JAK-1/STAT-3 pathway. This natural compound induces cell death and reduces tumor growth by blocking STAT-3 translocation.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Prostate cancer (PCA) is a prevalent malignancy affecting men.
  • Understanding PCA growth pathways is crucial for developing new therapies.
  • Ganoderma lucidum's anticancer potential against PC-3 cells via the JAK-1/STAT-3 pathway was investigated.

Purpose of the Study:

  • To evaluate the anticancer effects of Ganoderma lucidum on prostate cancer (PC-3) cells.
  • To investigate the role of the JAK-1/STAT-3 signaling pathway in Ganoderma lucidum's mechanism of action.
  • To determine if Ganoderma lucidum can inhibit prostate cancer cell proliferation and induce apoptosis.

Main Methods:

  • Cytotoxicity was assessed using the MTT assay.
  • Reactive oxygen species (ROS) production was monitored via DCFH-DA staining.
  • Apoptosis, protein expression (Bcl-2, JAK-1, STAT3, Bax, CyclinD1), and caspase activity were analyzed.

Main Results:

  • Ganoderma lucidum demonstrated significant cytotoxicity, ROS production, and apoptosis induction in PC-3 cells.
  • The compound inhibited STAT-3 translocation, a key factor in cell proliferation and apoptosis.
  • Downregulation of Bcl-2, cyclin-D1, and caspase-3/9, along with altered Bax expression, was observed.

Conclusions:

  • Ganoderma lucidum effectively inhibits prostate cancer cell growth.
  • Apoptosis is induced through the inhibition of STAT-3 translocation by Ganoderma lucidum.
  • This study highlights Ganoderma lucidum as a potential therapeutic agent for prostate cancer.
Keywords:
ApoptosisCyclin-D1Ganoderma lucidumPC-3 cellsProstate cancerSTAT-3 pathway

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