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Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

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PMAA-CeO₂ nanoparticle-based paper microfluidic device with customized image processing software for antioxidant

Abhay Sachdev^1,2, Pradipta Samanta^3,4, Vijayesh Kumar³

¹Ubiquitous Analytical Techniques Division, CSIR-Central Scientific Instruments Organization (CSIR-CSIO), Chandigarh, 160030, India. abhay.sachdev@csio.res.in.

Analytical and Bioanalytical Chemistry

|September 30, 2020

Summary

Researchers developed a simple microfluidic paper-based analytical device (μPAD) using nail polish for antioxidant testing. This low-cost, portable device utilizes nanoparticles to detect antioxidant activity in samples like tea, offering a reliable on-site assay method.

Keywords:

Antioxidant activity Cerium oxide Colorimetric Image processing software Nail paint μPAD

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Area of Science:

Analytical Chemistry
Materials Science
Nanotechnology

Background:

Microfluidic paper-based analytical devices (μPADs) are advancing, yet simple, customizable designs for antioxidant assays are needed.
Existing methods may lack portability, cost-effectiveness, or integrated imaging for real-time analysis.

Purpose of the Study:

To develop a facile and efficient μPAD fabrication method for antioxidant assays.
To create a μPAD with customized imaging capabilities for quantitative antioxidant determination.
To validate the developed μPAD for on-site antioxidant activity assessment in various samples.

Main Methods:

Fabrication of μPADs using transparent nail paint for hydrophobic barriers and channels.
Impregnation of μPADs with poly(methacrylic acid) (PMAA)-coated cerium oxide (CeO₂) nanoparticles.
Colorimetric detection of antioxidant activity via TMB oxidation, quantified using MATLAB-based image processing.

Main Results:

The fabricated μPADs exhibited well-defined channels and suitable resolution for assays.
A dose-dependent reduction in blue color intensity correlated with antioxidant concentration.
The μPAD demonstrated good analytical performance, stability, and resistance to interferents, performing comparably to standard methods in tea sample analysis.

Conclusions:

The developed nail paint-based μPAD offers a simple, low-cost, and portable platform for antioxidant assays.
The integrated nanoparticle-based colorimetric detection and image processing enable reliable on-site antioxidant activity determination.
This method shows significant potential for various point-of-care and field applications requiring antioxidant analysis.