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Changes in intracellular NAD status affect stomatal development in an abscisic acid-dependent manner
Elias Feitosa-Araujo1,2, Paula da Fonseca-Pereira1, Mateus M Pena1
1Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-900, Brazil.
The Plant Journal : for Cell and Molecular Biology
|September 30, 2020
Summary
Nicotinamide adenine dinucleotide (NAD) negatively impacts plant stomatal development. Maintaining intracellular NAD homeostasis is crucial for normal stomatal formation, linking metabolism to hormone signaling.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Nicotinamide adenine dinucleotide (NAD) is vital for metabolism and cell signaling.
- NAD influences stress responses and development.
- Its role in plant stomatal development is largely unexplored.
Purpose of the Study:
- To investigate the impact of NAD on stomatal development in Arabidopsis thaliana.
- To elucidate the mechanisms by which NAD affects stomatal formation.
Main Methods:
- Genetic manipulation of NAD+ transport and breakdown pathways.
- Analysis of stomatal density and lineage cell numbers.
- Gene expression analysis of stomatal development and ABA signaling pathways.
- In vivo measurement of ABA dynamics using ABAleon2.1 reporter.
Main Results:
- Reduced NAD+ transport or breakdown capacity led to decreased stomatal density.
- NAD+ treatment also reduced stomatal number and repressed stomatal lineage gene expression.
- Impaired NAD+ transport induced ABA-responsive genes, and ABA synthesis inhibition rescued the phenotype.
- NAD+ treatment increased ABA content, indicating NAD+ acts upstream of ABA signaling.
Conclusions:
- Intracellular NAD+ homeostasis is essential for normal stomatal development in Arabidopsis.
- NAD+ influences stomatal development by modulating ABA synthesis and signaling.
- This study links central metabolism, hormone signaling, and developmental plasticity in plants.
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