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Development of Rabbit Monoclonal Antibodies for Quantitation of Therapeutic Human Antibodies in Preclinical Non-Human

Ruiyin Chu1, Jacob Gerstein1, Hai Wu2

  • 1Biomarker and Clinical Bioanalysis, Sanofi North America, Framingham, Massachusetts, USA.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|September 30, 2020
PubMed

During preclinical studies, there is a great need to develop monoclonal antibodies (mAbs) that are specific to human immunoglobulin (IgG), without binding to monkey IgG, to detect therapeutic human mAb in non-human primates. We took advantage of the latest rabbit B cell cloning technology to develop six unique rabbit anti-human IgG mAb clones for this purpose. These clones are capable of binding to both human IgG and Fab with high affinity without nonspecific binding to cynomolgus monkey IgG. These clones have been evaluated as a generic capture reagent for the detection of human IgG and Fab, in the presence of cynomolgus monkey serum, by Gyrolab™ immunoassay. They may be used in singlet or as pairs for the detection of human IgG, in any host animal, to meet the need for therapeutic mAb development in preclinical studies.

Keywords:
epitope mappinggeneric pharmacokinetic assaymonoclonal antibodyrabbit B cell cloning

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