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Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
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Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet
Antonín Hlaváček1, Jana Křivánková, NaděŽda Pizúrová
1Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic. hlavacek@iach.cz.
The Analyst
|September 30, 2020
Abstract:
We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-d-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.

