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Serum citrullinated histone H3 concentrations differentiate patients with septic verses non-septic shock and

Yuzi Tian1,2, Rachel M Russo1, Yongqing Li3

  • 1Department of Surgery, University of Michigan Health System, University of Michigan Medical School, 1500 E Medical Center Dr. SPC 5331, Ann Arbor, MI, 48109-5331, USA.

Infection
|October 1, 2020
PubMed
Abstract

Insights

Levels of citrullinated histone H3 (CitH3) are elevated in septic shock patients, correlating with disease severity and poor prognosis. This finding offers insights into sepsis pathophysiology and potential diagnostic markers.

Area of Science:

  • Biochemistry
  • Immunology
  • Critical Care Medicine

Background:

  • Microbial infections trigger extracellular trap formation by immune cells, releasing citrullinated histone H3 (CitH3).
  • Peptidylarginine deiminase (PAD) 2 and 4 enzymes catalyze CitH3 formation.
  • Understanding these mechanisms is crucial for developing diagnostics and treatments for septic shock.

Purpose of the Study:

  • To determine CitH3 expression in septic shock patients.
  • To correlate CitH3 levels with PAD2/PAD4 expression.
  • To assess the relationship between CitH3 and clinical outcomes in septic shock.

Main Methods:

  • Serum samples from 160 critically ill patients (septic shock, non-infectious shock) and healthy volunteers were analyzed for CitH3 levels.
  • Clinical and laboratory characteristics of patients were assessed.
  • CitH3 levels were correlated with PAD2/PAD4 concentrations and Sequential Organ Failure Assessment (SOFA) scores.

Main Results:

  • Septic shock patients exhibited significantly higher circulating CitH3 levels compared to non-infectious shock patients.
  • CitH3 levels positively correlated with PAD2, PAD4, and SOFA scores.
  • Elevated CitH3 levels at 24 and 48 hours were associated with non-survival in septic patients.

Conclusions:

  • CitH3 is significantly increased in patients with septic shock.
  • Serum CitH3 concentrations correlate with disease severity and prognosis in sepsis.
  • CitH3 may provide valuable insights into sepsis pathophysiology and serve as a potential biomarker.
Keywords:
Citrullinated histone H3DiagnosisOutcomesPeptidylarginine deiminaseSepsis

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