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Updated: Dec 7, 2025
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
Published on: December 8, 2013
A cytosolically localized far-red to near-infrared rhodamine-based fluorescent probe for calcium ions
Koji Numasawa1, Kenjiro Hanaoka, Takayuki Ikeno
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@mol.f.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
Abstract:
Ca2+ is one of the most important second messengers in cells. A far-red to near-infrared (NIR) Ca2+ fluorescent probe is useful for multi-color imaging in GFP or YFP-expressing biosamples. Here we developed a cytosolically localized far-red to NIR rhodamine-based fluorescent probe for Ca2+, CaSiR-2 AM, while rhodamine dyes are basically localized to mitochondria or lysosomes in cells.
