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Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: Dec 7, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

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Correction to Light-Inducible Recombinases for Bacterial Optogenetics

Michael B Sheets^1,2, Wilson W Wong^1,2, Mary J Dunlop^1,2

¹Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, United States.

ACS Synthetic Biology

|October 1, 2020

Summary

No abstract available in PubMed .

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