Related Experiment Video
Updated: Dec 7, 2025
Innervation of Human Intestinal Organoids
Published on: January 17, 2025
Development of Colonic Organoids Containing Enteric Nerves or Blood Vessels from Human Embryonic Stem Cells
Chul Soon Park1, Le Phuong Nguyen1,2, Dongeun Yong1,2
1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, College of Medicine, Yonsei University, Seoul 03722, Korea.
Abstract:
The increased interest in organoid research in recent years has contributed to an improved understanding of diseases that are currently untreatable. Various organoids, including kidney, brain, retina, liver, and spinal cord, have been successfully developed and serve as potential sources for regenerative medicine studies. However, the application of organoids has been limited by their lack of tissue components such as nerve and blood vessels that are essential to organ physiology. In this study, we used three-dimensional co-culture methods to develop colonic organoids that contained enteric nerves and blood vessels. The development of enteric nerves and blood vessels was confirmed phenotypically and genetically by the use of immunofluorescent staining and Western blotting. Colonic organoids that contain essential tissue components could serve as a useful model for the study of colon diseases and help to overcome current bottlenecks in colon disease research.
Insights
Researchers developed new colonic organoids containing essential nerves and blood vessels. These advanced organoids offer a better model for studying colon diseases and advancing regenerative medicine.
Area of Science:
- Biomedical Engineering
- Developmental Biology
- Gastroenterology
Background:
- Organoid research has advanced disease understanding and regenerative medicine.
- Existing organoids (kidney, brain, liver) lack crucial tissue components like nerves and blood vessels.
- This limitation hinders their application in studying organ physiology and disease.
Purpose of the Study:
- To develop functional colonic organoids incorporating enteric nerves and blood vessels.
- To establish a more physiologically relevant model for colon disease research.
- To overcome current limitations in organoid applications for regenerative medicine.
Main Methods:
- Utilized three-dimensional co-culture techniques.
- Developed colonic organoids integrated with enteric nerves and blood vessels.
- Confirmed development using immunofluorescent staining and Western blotting.
Main Results:
- Successfully generated colonic organoids containing enteric nerves and blood vessels.
- Validated the presence and development of these critical tissue components.
- Demonstrated the potential of these enhanced organoids as a disease model.
Conclusions:
- Developed novel colonic organoids with integrated neurovasculature.
- These organoids represent a significant advancement for colon disease modeling.
- Offers a promising avenue for regenerative medicine and overcoming research bottlenecks.
More Related Videos
08:13Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids
Published on: July 9, 2021
10:39Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Published on: April 7, 2023