Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fecal microbiome of patients with ulcerative colitis reflects their phenotype and inflammatory level.

Scientific reports·2026
Same author

Discovery of a secreted <i>Bacteroides fragilis</i> mucinase that cleaves mucins with bis-T O-glycans through a carbohydrate binding module-dependent mechanism.

Gut microbes·2026
Same author

Taxonomic composition and carbohydrate-active enzyme content in microbial enrichments from pulp mill anaerobic granules after cultivation on lignocellulosic substrates.

Frontiers in microbiomes·2026
Same author

Phenogenomics reveals the ecology and evolution of Trichoderma fungi for sustainable agriculture.

Nature microbiology·2026
Same author

Microbial Biofilms Dynamics and Functionality in an Urban <i>Mycobacterium</i>-Dominated Drinking Water Distribution System.

Environmental science & technology·2026
Same author

Dual-Activity Mannosyltransferase Phosphorylases in Family 130 of Glycoside Hydrolases.

ACS catalysis·2026

Related Experiment Video

Updated: Dec 7, 2025

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

4.1K

Investigating host-microbiome interactions by droplet based microfluidics.

Alexandra S Tauzin1, Mariana Rangel Pereira2,3, Liisa D Van Vliet2,4

  • 1TBI, CNRS, INRAE, INSAT, Université de Toulouse, F-31400, Toulouse, France.

Microbiome
|October 2, 2020
PubMed
Summary
This summary is machine-generated.

A new droplet microfluidics method enables rapid screening of bacterial gut colonization pathways. This approach identifies novel targets for studying inflammatory bowel diseases and host-microbiota interactions.

Keywords:
Beta-N-acetyl-galactosaminidaseDroplet microfluidicsFunctional metagenomicsHuman glycansHuman gut microbiota

More Related Videos

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

4.3K

Related Experiment Videos

Last Updated: Dec 7, 2025

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

4.1K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.7K
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

4.3K

Area of Science:

  • Microbiology
  • Genomics
  • Biotechnology

Background:

  • Understanding bacterial gut colonization mechanisms is crucial for gut homeostasis.
  • Limited tools exist for studying uncultured microbes and their glycan foraging strategies.

Purpose of the Study:

  • To develop an efficient discovery tool for bacterial gut colonization mechanisms.
  • To identify novel pathways involved in host-microbiota interactions, particularly in inflammatory bowel diseases.

Main Methods:

  • Ultra-high-throughput screening using droplet microfluidics and fosmid libraries.
  • Screening for β-N-acetylgalactosaminidase activity in mucosal microbiota.
  • Utilizing a commercial flow cytometer for enzyme activity detection.

Main Results:

  • Identified pathways for degrading human gangliosides and milk oligosaccharides.
  • These pathways are structurally similar to intestinal mucin glycans.
  • Prevalence of these pathways is associated with inflammatory bowel diseases and may involve horizontal gene transfer from Bacteroides species.

Conclusions:

  • Droplet microfluidics significantly accelerates and miniaturizes screening of metagenomic libraries.
  • The developed method is versatile for various (meta)genomic libraries and enzyme activity detection.
  • This workflow advances functional metagenomics and holobiomics for deciphering host-microbiota relationships.