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Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
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A most formidable arsenal: genetic technologies for building a better mouse
James F Clark1, Colin J Dinsmore1, Philippe Soriano1
1Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mt. Sinai, New York, New York 10029, USA.
Genes & Development
|October 2, 2020
Summary
Mice are crucial for genetic research, with genome editing tools evolving from classic methods to advanced CRISPR technology. This review details these advancements and their impact on mouse genetics and allele engineering.
Area of Science:
- Genetics
- Molecular Biology
- Genomics
Background:
- Mice are a cornerstone model organism in genetic research.
- Genome modification tools have evolved significantly over decades.
- Classic techniques include forward screens and gene targeting in stem cells.
Purpose of the Study:
- To review the historical development of mouse genome modification techniques.
- To highlight the impact of recent CRISPR technology on mouse genetics.
- To survey common allele classes and their engineering methods.
Main Methods:
- Historical review of genetic tools.
- Analysis of nuclease-mediated genome editing techniques.
- Survey of mouse allele classes and engineering strategies.
Main Results:
- The evolution of mouse genetic tools spans from early screens to sophisticated CRISPR systems.
- Nuclease-mediated techniques are revolutionizing mouse genetics research.
- A comprehensive overview of allele types and their engineering is presented.
Conclusions:
- Understanding the historical and current landscape of mouse genome engineering is vital.
- CRISPR and other nucleases offer powerful new avenues for genetic studies in mice.
- This review provides a framework for selecting and engineering alleles for diverse research applications.
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