Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.5K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.5K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.3K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.3K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

3.8K
3.8K
Conserved Binding Sites01:49

Conserved Binding Sites

4.9K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.9K
Conserved Binding Sites01:49

Conserved Binding Sites

1.8K
1.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.3K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel CDK-independent function of CDC25 phosphatases in mRNA translation.

EMBO reports·2026
Same author

The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell model.

bioRxiv : the preprint server for biology·2026
Same author

Multi-omic screening identifies RBMXL3 as a primate-specific RNA-binding protein and candidate regulator of RNA metabolism in human spermatogenesis.

Cellular & molecular biology letters·2026
Same author

Correction: Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.

Molecular neurodegeneration·2026
Same author

Polysomal Profiling Coupled to Allele-Specific Proteomics Reveals an EIF4H TranSNP Allele Possessing Higher mRNA Translation Potential.

Molecular & cellular proteomics : MCP·2026
Same author

Nicotinamide N-Methyl Transferase (NNMT) Sustains Innate Sensitivity to NAMPT Inhibition in YAP-dependent Stem-like/Mesenchymal Prostate Cancer.

International journal of biological sciences·2026

Related Experiment Video

Updated: Dec 7, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.6K

Hyperconserved Elements in Human 5'UTRs Shape Essential Post-transcriptional Regulatory Networks.

Paola Zuccotti1, Daniele Peroni1, Valentina Potrich1

  • 1Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Frontiers in Molecular Biosciences
|October 2, 2020
PubMed
Summary

Researchers identified novel conserved elements in 5' untranslated regions (UTRs) that regulate gene expression. These elements, termed 5'HCEs, are crucial for controlling essential genes, including homeotic genes, via post-transcriptional regulation (PTR).

Keywords:
5cpsdummy′UTRRBMXRNA-binding proteinshomeoboxphylogenetic conservationpost-transcriptional regulationregulatory networks

More Related Videos

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.0K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.8K

Related Experiment Videos

Last Updated: Dec 7, 2025

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.6K
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.0K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Post-transcriptional regulation (PTR) significantly influences cellular phenotypes by controlling gene expression.
  • The 5' untranslated regions (UTRs) of mRNA are critical for PTR but remain less understood than 3'UTRs.
  • RNA-binding proteins (RBPs) and non-coding RNAs interact with UTRs to mediate PTR.

Purpose of the Study:

  • To identify and characterize functional elements within human 5'UTRs that are targets of PTR.
  • To investigate the role of these elements, particularly in the regulation of homeotic genes.
  • To establish a link between conserved 5'UTR elements and gene regulatory networks.

Main Methods:

  • Utilized a computational approach based on phylogenetic conservation to identify hyperconserved elements in human 5'UTRs (5'HCEs).
  • Analyzed the distribution, characteristics, and gene enrichment of identified 5'HCEs.
  • Integrated computational predictions with experimental validation to identify key RBPs and regulatory pathways.

Main Results:

  • Discovered over 5000 5'HCEs in approximately 10% of human protein-coding genes.
  • Found that 5'HCEs are short, often clustered elements, enriched in genes with essential cellular functions, including 20% of homeotic genes.
  • Identified RBMX as a key RBP initiating a PTR cascade that regulates numerous homeotic genes.

Conclusions:

  • Established 5'HCEs as evolutionarily conserved and functionally important sites for PTR in 5'UTRs.
  • Demonstrated that 5'HCEs are critical mediators of PTR networks, particularly impacting the expression of homeotic genes.
  • Highlighted the significance of 5'UTR regulation in developmental processes controlled by homeotic genes.