Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Iron overload disrupts bone homeostasis via TfR1-dependent ferroptosis and cGAS/STING-driven pyroptosis in pyogenic spondylitis.

Frontiers in immunology·2026

Same author

Machine Learning for Preoperative Prediction of Intraoperative Hypothermia in Gynecological Laparoscopic Surgery: A Retrospective Cohort Study.

The journal of obstetrics and gynaecology research·2026

Same author

Habitat-Based MRI Radiomics for Predicting Breast-Conserving Surgery Feasibility After Neoadjuvant Chemotherapy in Breast Cancer: A Multicenter Study.

Clinical breast cancer·2026

Same author

Machine learning for prediction of histologic chorioamnionitis (stage ≥II) in parturients receiving labor analgesia: a retrospective multicentre cohort study.

Frontiers in medicine·2026

Same author

Interstitial Copper Doping and Thermally Activated Rattling in La<sub>3-</sub> <sub>x</sub>Te<sub>4</sub> for Enhanced Thermoelectric Performance.

Angewandte Chemie (International ed. in English)·2026

Same author

Glymphatic system bridges peripheral and central nervous system changes in classic trigeminal neuralgia.

Brain communications·2026

Search research articles

Related Experiment Video

Updated: Dec 7, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A microfluidic chip for screening high-producing hybridomas at single cell level.

Weikai Zhang¹, Ren Li, Fei Jia

¹Department of Biomedical Engineering, School of Life Science, Beijing Institute of Technology, Beijing 100081, China. weizewen@bit.edu.cn liqin@bit.edu.cn.

|October 2, 2020

Summary

This study introduces a novel microfluidic chip for screening hybridomas, improving monoclonal antibody (mAb) production. The chip streamlines multiple screening steps, enhancing accuracy and reducing costs for hybridoma cell line development.

More Related Videos

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Related Experiment Videos

Last Updated: Dec 7, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Area of Science:

Biotechnology
Immunology
Microfluidics

Background:

Hybridomas are essential for monoclonal antibody (mAb) production in research and therapy.
Long-term hybridoma cultures lead to population heterogeneity and reduced antibody production.
Current screening methods are laborious and inefficient, often performed in multi-well plates.

Purpose of the Study:

To develop a novel microfluidic chip for efficient, single-cell level screening of hybridomas.
To overcome the limitations of traditional multi-well plate screening methods for hybridoma selection.
To improve the accuracy and monoclonality of monoclonal antibody production.

Main Methods:

Development of a single microfluidic chip integrating hybridoma trapping, proliferation, and transfer.
Incorporation of fluorescent identification for single-cell protein-antibody binding analysis.
Two-round screening of anti-CD45 mAb-secreting hybridomas using the microfluidic platform.

Main Results:

The microfluidic chip successfully screened high-producing hybridomas with minimal cell loss.
Demonstrated significant reduction in human labor and time costs compared to conventional methods.
Achieved enhanced accuracy and confirmed monoclonality in the selected hybridoma population.

Conclusions:

The novel microfluidic chip offers a comprehensive solution for multi-round hybridoma screening.
This technology enhances efficiency, accuracy, and monoclonality in monoclonal antibody production.
The platform is suitable for both laboratory studies and pilot-scale production of therapeutic mAbs.