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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

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Intensified Downstream Processing of Monoclonal Antibodies Using Membrane Technology.

Sathish Nadar¹, Gary Shooter¹, Balaji Somasundaram²

¹Australian Research Council Training Centre for Biopharmaceutical Innovation, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Corner College and Cooper Roads, Brisbane, Queensland, 4072, Australia.

Biotechnology Journal

|October 2, 2020

Summary

Membrane technologies are crucial for intensifying monoclonal antibody downstream processing. Their flexibility supports continuous and single-use bioprocessing, enhancing overall efficiency.

Keywords:

downstream processing membranes monoclonal antibody process integration process intensification

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Last Updated: Dec 7, 2025

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

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Published on: May 1, 2019

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

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Published on: January 2, 2017

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Published on: August 16, 2019

Area of Science:

Biotechnology
Chemical Engineering
Process Intensification

Background:

Advances in upstream monoclonal antibody (mAb) production necessitate parallel intensification of downstream processing.
Traditional downstream methods face challenges in meeting the demands of increased mAb titers.
The biopharmaceutical industry is shifting towards continuous and single-use manufacturing paradigms.

Purpose of the Study:

To review recent developments in membrane-based technologies for monoclonal antibody bioprocessing.
To highlight the role of membrane operations in integrating downstream processes.
To focus on the progress towards intensified end-to-end bioprocesses.

Main Methods:

Literature review of membrane-based unit operations in mAb downstream processing.
Analysis of recent advancements and applications of membrane technologies.
Evaluation of membrane technology's contribution to process integration and intensification.

Main Results:

Membrane technologies offer operational flexibility and ease of implementation, aligning with industry needs.
Integration of membrane unit operations is a key strategy for streamlining downstream processing.
Significant progress has been made in applying membrane technologies for intensified mAb production.

Conclusions:

Membrane technologies are essential for intensifying downstream processing of monoclonal antibodies.
These technologies are vital for enabling continuous and single-use bioprocessing strategies.
Further development and implementation of membrane-based solutions will drive the achievement of intensified end-to-end bioprocesses.