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Method agreement between three different chambers for comparative bull semen computer assisted sperm motility

A Basioura1, G Tsousis1, C Boscos1

  • 1Unit of Biotechnology of Reproduction, Farm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 11, St. Voutyra str., 54627, Thessaloniki, Greece.

Polish Journal of Veterinary Sciences
|October 2, 2020
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Summary

Computer-assisted sperm analysis (CASA) of bull semen shows that the analysis chamber significantly impacts results. Different chambers yield varying motility and kinematic values, affecting data comparability between labs.

Keywords:
analysis chamberbull semencomputer assisted sperm analysismotility

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Area of Science:

  • Veterinary Reproduction
  • Animal Breeding
  • Spermatozoa Analysis

Background:

  • Computer-assisted sperm analysis (CASA) is crucial for evaluating semen quality.
  • Standardization of CASA methods is essential for reliable results.
  • Semen analysis chambers may influence CASA outcomes.

Purpose of the Study:

  • To compare computer-assisted sperm analysis (CASA) results for frozen-thawed bull semen using three distinct chambers.
  • To assess the impact of chamber type on various semen parameters.

Main Methods:

  • Sixty frozen bull semen samples were thawed and prepared.
  • Semen was analyzed using CASA with Makler® chamber (MC; 10 μm), Leja 4 chamber slide (LC; 20 μm), and Glass slide with coverslip (GSC; 10.3 μm).
  • Parameters analyzed included total motility, progressive motility, VCL, VSL, VAP, ALH, BCF, LIN, STR, WOB, and hyperactive spermatozoa.

Main Results:

  • The Makler chamber yielded higher values for most parameters compared to LC and GSC.
  • No systematic differences were observed between LC and GSC for several kinematic parameters.
  • Method agreement varied: moderate between MC and LC, poor between MC and GSC, and moderate to good between LC and GSC.

Conclusions:

  • The choice of analysis chamber significantly influences CASA results for bull semen.
  • Discrepancies in CASA outcomes due to different chambers must be considered when comparing data across laboratories.
  • Standardization of chamber use in CASA is recommended for improved data consistency.