Related Experiment Video
Updated: Dec 7, 2025
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Evidence of new sequence types of methicillin-resistant Staphylococcus pseudintermedius in Italy
F P Nocera1, A Parisi2, M Corrente3
1Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy.
Abstract:
Staphylococcus pseudintermedius (SP) and methicillin-resistant SP (MRSP) is one of the most important veterinary pathogens in the dog. Herein, from a total of 126 S. pseudintermediusstrains, 23 MRSP (18%) were identified. Multi-Locus Sequence Typing (MLST) revealed that most of MRSP strains belonged to ST71 (26%), which have been already reported in Italy and other countries. Interestingly, nine new sequence types (39%), from 1053 up to 1061, were described for the first time. Moreover, the isolated MRSP strains showed relevant antibiotic resistance profiles. This report highlights the circulation of new sequence types of MRSP in Italy and underlines the need of a global epidemiological surveillance to limit the increasing spread of multidrug-resistant MRSPstrains worldwide, since they may represent a considerable concern for dog's health.
Insights
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a significant veterinary pathogen in dogs. This study identified new MRSP sequence types in Italy, highlighting the need for global surveillance to control multidrug-resistant strains.
Area of Science:
- Veterinary Microbiology
- Epidemiology
- Antimicrobial Resistance
Background:
- Staphylococcus pseudintermedius (SP) is a major veterinary pathogen in dogs.
- Methicillin-resistant SP (MRSP) poses a significant threat to canine health.
- Understanding the genetic diversity and spread of MRSP is crucial for effective control strategies.
Purpose of the Study:
- To identify and characterize MRSP strains in dog populations.
- To determine the genetic relatedness of MRSP strains using Multi-Locus Sequence Typing (MLST).
- To assess the antibiotic resistance profiles of isolated MRSP strains.
Main Methods:
- Isolation and identification of Staphylococcus pseudintermedius from canine samples.
- Detection of methicillin resistance using antimicrobial susceptibility testing.
- Multi-Locus Sequence Typing (MLST) for genotypic characterization of MRSP strains.
- Analysis of antibiotic resistance patterns.
Main Results:
- Out of 126 S. pseudintermedius strains, 23 (18%) were identified as MRSP.
- The most prevalent MRSP sequence type (ST) was ST71 (26%).
- Nine novel MRSP sequence types (ST 1053-1061) were identified for the first time (39%).
- Isolated MRSP strains exhibited concerning antibiotic resistance profiles.
Conclusions:
- This study highlights the emergence of new MRSP sequence types in Italy.
- The findings underscore the importance of ongoing global epidemiological surveillance for multidrug-resistant MRSP.
- Effective surveillance is essential to mitigate the spread of MRSP and protect dog health worldwide.
