Immunophenotyping reveals distinct subgroups of lupus patients based on their activated T cell subsets

This study performed an integrated analysis of the cellular and transcriptional differences in peripheral immune cells between patients with Systemic Lupus Erythematosus (SLE) and healthy controls (HC).

Peripheral blood was analyzed using standardized flow cytometry panels. Transcriptional analysis of CD4⁺ T cells was performed by microarrays and Nanostring assays.

SLE CD4⁺ T cells showed an increased expression of oxidative phosphorylation and immunoregulatory genes. SLE patients presented higher frequencies of activated CD38⁺HLA-DR⁺ T cells than HC. Hierarchical clustering identified a group of SLE patients among which African Americans were overrepresented, with highly activated T cells, and higher frequencies of Th1, Tfh, and plasmablast cells. T cell activation was positively correlated with metabolic gene expression in SLE patients but not in HC.

SLE subjects presenting with activated T cells and a hyperactive metabolic signature may represent an opportunity to correct aberrant immune activation through targeted metabolic inhibitors.