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Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome
Yu-Wei Yang1, Lu Chen1, Qiao Mou1
1College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Theriogenology
|October 2, 2020
Summary
Vitamin C (ascorbic acid) boosts male pig reproductive cells by promoting growth and reducing cell death. This vitamin influences gene expression and hormone secretion, enhancing overall reproductive function.
Area of Science:
- Reproductive Biology
- Cell Biology
- Biochemistry
Background:
- Vitamin C (ascorbic acid, AA) is known for its antioxidant properties and role in cellular processes.
- The impact of AA on male animal reproduction is not well understood.
- Sertoli cells are crucial for male reproductive function.
Purpose of the Study:
- To investigate the effects of exogenous Vitamin C (ascorbic acid) supplementation on porcine immature Sertoli cells (iSCs).
- To explore the molecular mechanisms by which AA influences iSC proliferation, apoptosis, and reproductive function.
Main Methods:
- Porcine immature Sertoli cells (iSCs) were treated with 250 μM AA for 36 hours.
- Transcriptome sequencing (mRNA and lncRNA) was performed to identify differentially expressed genes.
- Gene enrichment analysis and biochemical assays were conducted.
Main Results:
- AA supplementation promoted iSC proliferation, suppressed apoptosis, and decreased global nucleic acid methylation.
- Transcriptome analysis revealed 1232 differentially expressed mRNAs and 937 lncRNAs.
- AA elevated the secretion of anti-Müllerian hormone, inhibin B, and lactate, and enhanced lactate dehydrogenase activity.
Conclusions:
- Vitamin C (ascorbic acid) enhances the reproductive function of pig immature Sertoli cells.
- AA may exert its effects by reprogramming the global transcriptome and modulating hormone secretion and metabolite production.
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