Innovative Tools and Strategies for Optimizing Yeast Cell Factories
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Updated: Sep 20, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Gregory Guirimand1, Natalja Kulagina2, Nicolas Papon3
1Graduate School of Sciences, Technology and Innovation, Kobe University, Kobe, Japan; Biomolécules et Biotechnologies Végétales (BBV), Équipe d'Accueil (EA) 2106, Université de Tours, Tours, France.
Abstract:
Metabolic engineering (ME) aims to develop efficient microbial cell factories that can produce a wide variety of valuable compounds, ideally at the highest yield and from various feedstocks. We summarize recent developments in ME methods for tailoring different yeast cell factories (YCFs). In particular, we highlight the most timely and cutting-edge molecular tools and strategies for biosynthetic pathway optimization (including genome-editing tools), combinatorial transcriptional and post-transcriptional engineering (cis/trans regulators), dynamic control of metabolic fluxes (e.g., rewiring of primary metabolism), and spatial reconfiguration of metabolic pathways. Finally, we discuss challenges and perspectives for adaptive laboratory evolution (ALE) of yeast to advance ME of microbial cell factories.
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