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Long non-coding RNA X-inactive-specific transcript contributes to cisplatin resistance in gastric cancer by sponging

Xiao Han1, Hai-Bin Zhang, Xue-Di Li

  • 1Internal Medicine-Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China.

Anti-Cancer Drugs
|October 3, 2020
PubMed

Insights

X-inactive-specific transcript (XIST) is overexpressed in gastric cancer, promoting cisplatin resistance. This oncogenic noncoding RNA interacts with Let-7b-5p, impacting cancer cell survival and treatment outcomes.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • X-inactive-specific transcript (XIST) is a 19 kb noncoding RNA with oncogenic roles in various cancers, including gastric cancer.
  • XIST is implicated in cisplatin resistance in gastric cancer cells, but the underlying mechanisms are not fully understood.

Purpose of the Study:

  • To investigate the role of XIST in gastric cancer and its contribution to cisplatin resistance.
  • To identify potential molecular interactions involving XIST in gastric cancer.

Main Methods:

  • Quantification of XIST levels in gastric cancer cells and patient tumor specimens.
  • Bioinformatic prediction and experimental validation of microRNA interactions with XIST.
  • Assessment of the impact of XIST and microRNA modulation on gastric cancer cell behavior and gene expression.

Main Results:

  • XIST is overexpressed in gastric cancer cells and tumors, correlating with poor patient prognosis.
  • High XIST levels are associated with increased cisplatin resistance in gastric cancer cells.
  • Let-7b-5p was identified as a direct interaction partner of XIST, with an inverse correlation in tumor tissues.
  • Modulation of Let-7b-5p partially rescued the effects of XIST knockdown on cell proliferation and apoptosis via Aurora kinase B regulation.

Conclusions:

  • XIST acts as an oncogene in gastric cancer, conferring cisplatin resistance through its interaction with Let-7b-5p.
  • The XIST/Let-7b-5p axis represents a potential therapeutic target for overcoming cisplatin resistance in gastric cancer.

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