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Updated: Dec 7, 2025

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
Published on: October 6, 2022
A compendium of DNA-binding specificities of transcription factors in Pseudomonas syringae
Ligang Fan1,2, Tingting Wang2, Canfeng Hua2
1School of Medicine, Northwest University, 710069, Xi'an, China.
Abstract:
Pseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.
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