Related Experiment Video
Updated: Dec 7, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
The Cramer's rule for the parametrization of phenol and its hydroxylated byproducts: UV spectroscopy vs. high
Yesid S Murillo-Acevedo1, Liliana Giraldo2, Po S Poon3
1Departamento de Química, Facultad de Ciencias, Universidad de los Andes, Bogotá, Colombia.
Abstract:
A linear algebra theorem like Cramer's rule was used for the analysis of a system of equations obtained from UV spectroscopy, and results were compared against those obtained from HPLC analysis. This parametrization allowed to quantify the concentration of the main intermediate products detected along the photodegradation of phenol under UV-Vis irradiation of TiO2. UV spectroscopy data for phenol, hydroquinone, and benzoquinone were analyzed using the Cramer's rule. The overlapping interference of the intermediate products in the UV spectra was corrected. It can be concluded that the Cramer's rule can be used for the parametrization of the UV absorbance data of phenol and its main intermediate products. This methodology permitted to obtain the concentration of phenol and their intermediate products by UV-visible with a high precision in comparison of HPLC. The parametrization showed a correlation coefficient of ca. 0.9775 between the phenol concentration obtained by UV spectroscopy and values obtained from HPLC analysis. In this sense, results can be considered with good precision, and accordingly, it can be concluded that the methodology is reliable, and UV-visible spectroscopy can be selected instead of HPLC in much of the experiments concerning with aqueous-phase reactions.
More Related Videos
10:30A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
14:39Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Related Concept Videos
UV–Vis Spectroscopy: Woodward–Fieser Rules
Benzene to Phenol via Cumene: Hock Process
IR and UV–Vis Spectroscopy of Aldehydes and Ketones
Acidity and Basicity of Alcohols and Phenols
Structure and Nomenclature of Alcohols and Phenols
Alcohols are one of the most important functional groups in organic chemistry. The name of alcohol comes from the hydrocarbon from which it is derived. Alcohols are organic molecules containing the functional hydroxyl or –OH group directly bonded to carbon. Phenols have an OH group directly attached to a benzene ring. While alcohols are colorless, phenol is a white crystalline compound with a characteristic "hospital smell" odor.
As with other organic compounds, alcohols and...
Hydrolysis of Chlorobenzene to Phenol: Dow Process