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A yeast operator overlaps an upstream activation site.

J W Kronstad, J A Holly, V L MacKay

|July 31, 1987

Summary

This summary is machine-generated.

The BAR1 gene product in yeast a-cells inactivates alpha-factor. Its transcription is regulated by MAT alpha 2 protein and alpha-factor, impacting yeast mating and gene regulation.

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Area of Science:

Molecular Biology
Yeast Genetics
Gene Regulation

Background:

The BAR1 gene in Saccharomyces cerevisiae is expressed only in a-cells and encodes a protein that inactivates alpha-factor, a mating pheromone produced by alpha-cells.
The MAT alpha 2 protein, a key regulator in yeast mating, represses a-cell-specific genes, including BAR1, in alpha and a/alpha diploid cells.
BAR1 transcription in a-cells is induced upon exposure to alpha-factor, indicating a complex regulatory network.

Purpose of the Study:

To investigate the regulatory mechanisms controlling BAR1 gene expression in Saccharomyces cerevisiae.
To identify the specific DNA sequences responsible for MAT alpha 2-mediated repression and alpha-factor-induced activation of BAR1 transcription.
To understand the implications of these findings for the negative control of gene transcription in yeast.

Main Methods:

Deletion analysis of the 5' noncoding region of the BAR1 gene.
Analysis of gene transcription in response to specific mating factors and regulatory proteins.

Main Results:

Deletion analysis revealed that the major upstream activation site (UAS) of BAR1 overlaps with the 31 bp operator sequence essential for MAT alpha 2 repression.
The sequence TGAAACA was identified as mediating alpha-factor-stimulated transcription of BAR1.
These findings highlight a regulatory overlap crucial for controlling gene expression in yeast.

Conclusions:

The study elucidates the intricate regulation of BAR1 gene expression, involving both repression by MAT alpha 2 and activation by alpha-factor.
The overlapping regulatory elements for repression and activation have significant implications for understanding negative transcriptional control in yeast.
The identified TGAAACA sequence is a key mediator of pheromone-induced gene expression in a-cells.