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Cholera toxin induces food allergy through Th2 cell differentiation which is unaffected by Jagged2

Shanfeng Sun1, Songsong Jiang2, Junjuan Wang1

  • 1Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Life Sciences
|October 3, 2020
PubMed
Abstract

Insights

Cholera toxin promotes Th2 cell differentiation in food allergies, enhancing immune responses. This study reveals its adjuvant mechanism, offering insights into immune regulation and future research directions.

Area of Science:

  • Immunology
  • Allergy Research

Background:

  • Cholera toxin is utilized to induce food allergies, but its precise mechanism and effects are not fully understood.
  • Investigating the role of cholera toxin in immune regulation during food allergy is crucial.

Purpose of the Study:

  • To determine the molecular basis and signaling mechanisms of cholera toxin's immune regulation in a food allergy model.
  • To elucidate the adjuvant role of cholera toxin in food allergy development.

Main Methods:

  • Established a BALB/c mouse model of cholera toxin/ovalbumin-induced food allergy.
  • Analyzed Th1/Th2 cell differentiation and bone marrow-derived dendritic cells (BMDCs) response to cholera toxin in vitro.
  • Investigated the involvement of the Jagged2 Notch signaling pathway in Th cell differentiation.

Main Results:

  • Cholera toxin was found to promote Th2 cell differentiation and enhance immune responses in the presence of allergens.
  • Cholera toxin induced the expression of the Notch ligand Jagged2.
  • The Jagged2 Notch signaling pathway was not essential for cholera toxin-mediated differentiation of Th2 cells by BMDCs.

Conclusions:

  • This study provides initial insights into the adjuvant mechanism of cholera toxin in food allergy.
  • The findings offer a foundation for future research on cholera toxin's role in allergic immune responses.
Keywords:
AdjuvantCholera toxinFood allergyNotch signalingTh2 cell differentiation

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