Sulfur transformation in sulfur autotrophic denitrification using thiosulfate as electron donor

Thiosulfate is frequently used as an energy source and electron donor in autotrophic denitrification (AD) for removing nitrate from wastewater. However, transforming pathways of S₂O₃^2- in this process is unclear. Herein, the aim of this study is to explore possible transforming pathways of sulfur compounds in thiosulfate-based AD process. After measuring the variation of NO₃^-, NO₂^-, and various sulfur compounds such as S⁰, SO₄^2-, S₂O₃^2-, acid volatile sulfide (AVS), and S^2- in the presence and absence of S₂O₃^2-, the variation process of S₂O₃^2- and the contribution of various sulfur compounds were analyzed. The results indicated that S⁰, AVS, and S^2- were the intermediate products when S₂O₃^2- was applied as an electron donor. All S₂O₃^2-, S⁰, AVS, and S^2- could act as electron donors in the nitrate removal process with the final products of SO₄^2-. The utilization priority of these four sulfur sources was presumed in the following order: S^2- > S₂O₃^2- > AVS ≈ S⁰. Furthermore, sulfur transformation and balance in nitrate removal process was also investigated. This suggests the transforming pathways of sulfur compounds in denitrification process. Nitrogen removal and sulfur conversion process are dependent on the presence of microorganisms in the sludge.