Related Experiment Video
Updated: Aug 6, 2026
13:06
The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
Published on: December 15, 2012
14.6K
Genome editing systems across yeast species
1Department of Chemical & Biomolecular Engineering, Clemson University, Clemson, SC 29634, United States.
Current Opinion in Biotechnology
|October 4, 2020
Summary
Advanced genome editing tools are revolutionizing yeast cell factory development. This review covers traditional and novel techniques for efficient yeast engineering and phenotype screening.
Area of Science:
- Biotechnology
- Synthetic Biology
- Microbial Engineering
Background:
- Yeasts are crucial for producing valuable compounds.
- Efficient yeast cell factories rely on genome engineering tools.
- Traditional methods like homologous recombination are established but evolving.
Purpose of the Study:
- To review current and emerging genome editing techniques for yeast.
- To discuss their application in both model and non-conventional yeasts.
- To provide guidelines for adapting these tools across species.
Main Methods:
- Summarizing successful implementations of genome editing in Saccharomyces cerevisiae and other yeasts.
- Discussing mechanisms and applications of various genome engineering systems.
- Highlighting strategies for interspecies expansion of genome editing tools.
Main Results:
- Novel systems like CRISPR-Cas and base editors offer powerful, rapid genome engineering.
- Multiplexed approaches and bacteriophage integrases enhance engineering capabilities.
- Successful applications span model and diverse non-conventional yeast species.
Conclusions:
- Genome editing tools are essential for advancing yeast biotechnology.
- Emerging technologies significantly improve efficiency and scope of yeast engineering.
- Adaptability of these tools across yeast species is key for broader applications.
