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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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Updated: Dec 6, 2025

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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An Alternative Splicing Program for Mouse Craniofacial Development.

Joan E Hooper1, Kenneth L Jones2, Francis J Smith3

  • 1Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO, United States.

Frontiers in Physiology
|October 5, 2020
PubMed
Summary
This summary is machine-generated.

Alternative splicing significantly contributes to transcript diversity during mammalian facial development. Splicing patterns change more with tissue layer and age than facial prominence, highlighting its crucial role.

Keywords:
RNA binding proteinsectodermfacial developmentfacial prominencesmesenchymenasal epitheliumskipped exonsplicing regulators

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Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Alternative splicing is vital for generating functional transcripts and normal development.
  • Disruptions in splicing factors impact mammalian craniofacial development.
  • Systematic analysis of splicing dynamics during face formation is lacking.

Purpose of the Study:

  • To systematically analyze differential splicing dynamics during mouse facial development.
  • To investigate the influence of age, tissue layer, and facial prominence on splicing.
  • To identify the role of alternative splicing in craniofacial development.

Main Methods:

  • Deep RNA sequencing of developing mouse facial tissues (ectodermal and mesenchymal).
  • Analysis of samples from three facial prominences and nasal pit at embryonic days 10.5, 11.5, and 12.5.
  • Correlation analysis of splicing variations with RNA binding protein expression.

Main Results:

  • Alternative splicing contributes significantly to transcript diversity, comparable to differential gene expression.
  • Splicing changes are more prevalent across tissue layers and developmental time than between facial prominences.
  • Exon skipping was identified as the most common alternative splicing event.
  • Splicing variations correlate with RNA binding protein expression patterns.

Conclusions:

  • Alternative splicing is a major driver of transcript diversity during critical stages of mammalian facial development.
  • Splicing regulation is dynamic and influenced by tissue layer and age.
  • These findings define an alternative splicing regulatory program essential for normal craniofacial development.