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Related Experiment Video

Updated: Dec 6, 2025

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hUCMSCs Mitigate LPS-Induced Trained Immunity in Ischemic Stroke.

Yi-Wei Feng1,2, Cheng Wu3, Feng-Yin Liang1

  • 1Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Frontiers in Immunology
|October 5, 2020
PubMed
Summary

Innate immune memory worsens stroke outcomes by increasing inflammation and brain damage. Mesenchymal stem cell therapy reduces this detrimental memory, offering a new therapeutic approach for stroke patients.

Keywords:
H3K4me1innate immune memorymesenchymal stem cellmicrogliastroke

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Area of Science:

  • Neuroimmunology
  • Stem Cell Biology
  • Stroke Pathophysiology

Background:

  • Innate immune memory, a component of the innate immune system, aids pathogen clearance but can worsen neurological conditions.
  • Stroke is a significant cause of neuropathology, and the role of innate immune memory in its exacerbation is not fully understood.

Purpose of the Study:

  • To investigate the detrimental role of innate immune memory in stroke.
  • To determine if mesenchymal stem cell therapy can mitigate innate immune memory in the context of stroke.

Main Methods:

  • Activation of innate immune memory using lipopolysaccharide.
  • Administration of mesenchymal stem cell therapy post-activation.
  • Assessment of H3 methylation levels.
  • Evaluation of stroke outcomes, including ischemic infarct size.

Main Results:

  • Innate immune memory was found to be detrimental in stroke, promoting acute immune responses and increasing ischemic damage.
  • Mesenchymal stem cell therapy effectively diminished innate immune memory.
  • This diminution was associated with the down-regulation of H3 methylation.
  • Mesenchymal stem cell therapy provided protection against stroke.

Conclusions:

  • Innate immune memory plays a harmful role in stroke pathology.
  • Mesenchymal stem cell therapy demonstrates potential as a treatment for stroke by targeting and reducing innate immune memory.
  • Down-regulation of H3 methylation is a key mechanism by which mesenchymal stem cells exert their protective effects.