[Improved local hemolysis method for evaluating immunotropic agents].

Area of Science:

• Immunology
• Biotechnology

Background:

• The Jerne and Nordin method (1963) is a standard for detecting hemolytic activity.
• The Cunningham method (1965) offers an alternative approach to assessing hemolysis.

Purpose of the Study:

• To introduce a simplified and cost-effective modification of the Cunningham method for analyzing hemolysis zones.
• To evaluate the sensitivity and reproducibility of the proposed modification.

Main Methods:

• A modified chamber production technique was developed, emphasizing simplicity and reusability.
• The modified method incorporated a sealing support for incubation and counting of hemolysis zones.
• Two experimental series were conducted to compare the modification with the Jerne and Nordin method.

Main Results:

• The modified method demonstrated positive characteristics including ease of chamber production and reusability.
• The use of a sealing support facilitated incubation and counting of hemolysis zones.
• Sensitivity and reproducibility were assessed through experimental comparisons.

Conclusions:

• The proposed modification offers a practical and efficient alternative for hemolysis zone analysis.
• The technique's simplicity and reusability make it a valuable tool in immunological studies.
• Further validation confirmed its potential for reliable assessment of hemolytic activity.