Botrops derived hemocoagulase formulation a probable agent for diabetic wound healing.

Area of Science:

• Biomedical Science
• Wound Healing Research
• Pharmacology

Background:

• Diabetic wounds present a significant clinical challenge due to impaired healing.
• Botroclot, a hemocoagulase preparation from *Botrops atrox*, is used for minor surgical wounds but its efficacy in diabetic conditions is unknown.

Purpose of the Study:

• To investigate the efficacy of topical Botroclot in accelerating wound healing in a rat model of diabetes.

Main Methods:

• Diabetes was induced in rats using a high-fat diet and streptozotocin.
• Punch biopsy wounds were created and treated with topical Botroclot for 15 days.
• Wound closure, inflammatory markers, collagen deposition, and histopathology were assessed.

Main Results:

• Topical hemocoagulase application significantly improved wound closure rates.
• A notable decrease in inflammatory markers and a substantial increase in collagen deposition were observed.
• Histological analysis confirmed increased granulation tissue, indicating enhanced healing.

Conclusions:

• Botroclot demonstrates a mild to moderate positive effect on collagen deposition and wound contraction in diabetic rat wounds.
• This study supports the potential of venom-derived agents for improving diabetic wound healing.