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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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A Spellbinding Interplay Between Biological Barcoding and Nanotechnology.

Shehla Munir1, Sarfraz Ahmed2, Muhammad Ibrahim1

  • 1Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.

Frontiers in Bioengineering and Biotechnology
|October 5, 2020
PubMed
Summary
This summary is machine-generated.

Nano bio-barcodes offer advanced methods for identifying biomolecules and biodiversity. These technologies enhance disease diagnosis, drug delivery, and pathogen detection in various samples, promising future applications in environmental and clinical monitoring.

Keywords:
barcoded nanoparticlesbiobarcodingcytokinesnano-barcodingnano-biosensorsnanotechnologynucleic acidsproteins

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Advancements in scientific research are crucial for biological exploration.
  • Bio-barcodes and nanotechnology represent cutting-edge techniques for biomolecule analysis.
  • These technologies enable high-throughput screening for biodiversity and disease diagnostics.

Purpose of the Study:

  • To review current advancements in nano bio-barcodes.
  • To explore applications in multiplex detection of DNA, proteins, and nucleic acids.
  • To highlight potential uses in disease diagnosis, drug delivery, and environmental monitoring.

Main Methods:

  • Utilizes bio-barcoding techniques combined with nanotechnology.
  • Employs bio-barcode amplification assay (BCA) for detection.
  • Focuses on sensitive and rapid detection of various biological targets.

Main Results:

  • Demonstrates high sensitivity for uniplex and multiplex detection of pathogens.
  • Identifies applications in detecting cytokines, cancer markers, and allergens.
  • Shows promise for detecting fungi, algae, protozoa, and pollutants.

Conclusions:

  • Nano bio-barcodes provide powerful tools for molecular detection and identification.
  • These technologies offer significant potential for applications in clinical diagnostics, food safety, agriculture, and environmental monitoring.
  • Future development aims to create efficient sensors for swift detection of various analytes.