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Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
Published on: March 19, 2018
Platelet rich plasma-derived microvesicles increased in vitro wound healing
1Innovative Research Laboratory for Wound Healing, Health Sciences Department, University Eastern Piedmont, Novara, Italy. filippo.reno@med.uniupo.it.
Objective:
Platelet rich plasma (PRP) is a haemoderivative used in clinical practice for the treatment of hard-to-heal wounds. Platelet (PLT) activation is a key factor in the wound healing process leading to the production of extracellular vesicles. We obtained PRP and PRP-derived microvesicles (PLT-MVs) from healthy donors and compared their pro-healing efficacy in an in vitro wound model using human keratinocytes.
Materials And Methods:
We evaluated PLT-MVs' direct effect on an in vitro model of wound healing. PRP, PRP activated using calcimycin, and PLT-MVs separated by high speed centrifugation were added to scratched keratinocyte monolayers. Fluorescein diacetate was used in flow cytometry to distinguish PLTs and PLT-MVs from debris, and then, PLT-MVs were quantified on the basis of relative dimensions (Forward Scatter signals).
Results:
Wound areas were measured at time 0 and after 24 hours and they were healed by 24.80 ± 4.28% in control conditions, while PRP, activated PRP, and PLT-MVs increased closure of 62.94 ± 0.96%, 52.69 ± 17.20% and 52.76 ± 9.44%, respectively.
Conclusions:
Our results indicate that PRP pro-healing effects were fully replicable by PLT-MVs, suggesting a key role of microvesicles in the healing process and a possible clinical use as an alternative to PRP.
Insights
Platelet-rich plasma (PRP) and its derived microvesicles (PLT-MVs) significantly accelerate wound healing in keratinocytes. PLT-MVs show comparable pro-healing efficacy to PRP, suggesting their potential as a clinical alternative for wound treatment.
Area of Science:
- Biomedical Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Platelet-rich plasma (PRP) is a clinical tool for treating difficult wounds.
- Platelet activation is crucial for wound healing, producing extracellular vesicles.
- Platelet-derived microvesicles (PLT-MVs) are key mediators in this process.
Purpose of the Study:
- To compare the pro-healing efficacy of PRP and PLT-MVs.
- To investigate the role of PLT-MVs in keratinocyte wound healing.
- To assess PLT-MVs as a potential alternative to PRP in clinical applications.
Main Methods:
- PRP and PLT-MVs were isolated from healthy donors.
- An in vitro wound model using human keratinocytes was employed.
- Flow cytometry and cell monolayer scratching assays were utilized to evaluate healing.
Main Results:
- PRP and PLT-MVs significantly enhanced keratinocyte wound closure compared to controls.
- Wound closure increased by approximately 63% with PRP and 53% with PLT-MVs.
- PLT-MVs demonstrated comparable pro-healing effects to PRP.
Conclusions:
- PRP's wound healing benefits are largely attributable to PLT-MVs.
- PLT-MVs represent a promising cell-free therapeutic alternative to PRP.
- Further clinical studies are warranted to explore PLT-MVs for wound treatment.
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