Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.3K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.3K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

4.4K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.4K
Maturation of Endosomes01:28

Maturation of Endosomes

5.3K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
5.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.6K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.6K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

8.5K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.5K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

7.2K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NK cell dysfunction and interferon-γ production underlie autoinflammation in mevalonate kinase deficiency.

Immunity·2026
Same author

Fibroblastic reticular cells direct the initiation of T cell responses via CD44.

Nature·2026
Same author

Core fucosylation of IL-2RB is required for natural killer cell homeostasis.

Cell reports·2025
Same author

NK resident memory cells arise from NK cells that accumulate in tissues independently of persistent local infection.

Immunity·2025
Same author

DOT1L maintains NK cell phenotype and function for optimal tumor control.

Cell reports·2024
Same author

Tissue-resident memory NK cells: Homing in on local effectors and regulators.

Immunological reviews·2024
Same journal

Multi-Omics Comes of Age: Immune Profiling of Human Aging.

Immunology and cell biology·2026
Same journal

Mechanisms of Therapeutic Resistance and Recent Advances in Glioblastoma Treatment.

Immunology and cell biology·2026
Same journal

Ancestry Matters in Decoding Immunity.

Immunology and cell biology·2026
Same journal

Improving COVID-19 Vaccine Uptake Among Ethnic Minority Communities in Wales: A Community-Based Approach.

Immunology and cell biology·2026
Same journal

4-1BB Expression and Signaling Regulates MAIT Cell Activation and Effector Functions.

Immunology and cell biology·2026
Same journal

Highlights of 2025: Advances in the T-Cell Regulation of Germinal Center Response.

Immunology and cell biology·2026
See all related articles

Related Experiment Video

Updated: Dec 6, 2025

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.9K

NKG7 - regulating endosomal pathways?

Iona S Schuster1,2, Christopher E Andoniou1,2

  • 1Infection and Immunity Program and Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Immunology and Cell Biology
|October 5, 2020
PubMed
Summary
This summary is machine-generated.

Natural killer group 7 (NKG7) protein regulates inflammation and cytotoxic cell degranulation. NKG7 deficiency in mice impairs CD8 T cell responses and antigen presentation, suggesting a role in immune cell trafficking.

More Related Videos

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
05:13

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Published on: June 30, 2021

4.5K
In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.4K

Related Experiment Videos

Last Updated: Dec 6, 2025

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.9K
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
05:13

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Published on: June 30, 2021

4.5K
In Vitro Polymerization of F-actin on Early Endosomes
12:15

In Vitro Polymerization of F-actin on Early Endosomes

Published on: August 28, 2017

9.4K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Inflammation is a critical immune response.
  • Cytotoxic cells and CD8 T cells play key roles in adaptive immunity.
  • The precise regulators of these immune responses are still being elucidated.

Purpose of the Study:

  • To identify novel regulators of inflammation.
  • To investigate the role of NKG7 in immune cell function.
  • To understand the mechanism of NKG7 in immunological challenges.

Main Methods:

  • Analysis of NKG7-deficient mice.
  • Assessment of cytotoxic cell degranulation.
  • Evaluation of CD8 T cell expansion and trafficking.
  • Investigation of antigen presentation pathways.

Main Results:

  • NKG7 is identified as a regulator of inflammation.
  • NKG7 is required for cytotoxic cell degranulation.
  • NKG7 deficiency leads to reduced CD8 T cell expansion and trafficking.
  • Altered antigen presentation was observed in NKG7-deficient mice.

Conclusions:

  • NKG7 plays a significant role in regulating inflammatory responses.
  • NKG7 is crucial for optimal cytotoxic cell function and CD8 T cell-mediated immunity.
  • The mechanism may involve the regulation of endosomal vesicle trafficking.