Rufibacter latericius sp. nov., isolated from Baiyang Lake

A novel Gram-stain-negative, strictly aerobic, rod-shaped, brick red-pigmented bacterium, designated R-22-1 c-1^T, was isolated from water from Baiyang Lake, Hebei Province, PR China. The strain was able to grow at 20-30 °C (optimum, 30 °C) and pH 6-7 (optimum, pH 6) in Reasoner's 2A medium. 16S rRNA gene sequence and phylogenetic analyses of R-22-1 c-1^T revealed closest relationships to Rufibacter immobilis MCC P1^T (97.8 %), Rufibacter sediminis H-1^T (97.9 %) and Rufibacter glacialis MDT1-10-3^T (97.0 %), with other species of the genus Rufibacter showing less than 97.0 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids. The major cellular fatty acids were iso-C_15 : 0, C_15 : 1  ω6c, C_17 : 1  ω6c, anteiso-C_15 : 0, summed feature 3 (iso-C_15 : 0 2-OH and/or C_16 : 1  ω7c and/or C_16 : 1  ω6c) and summed feature 4 (iso-C_17 : 1I and/or anteiso-C_17 : 1B). The respiratory quinone was MK-7. The draft genome of R-22-1 c-1^T was 5.6 Mbp in size, with a G+C content of 50.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain R-22-1 c-1^T and related type strains were R. immobilis MCC P1^T (77.2 and 21.8 %), R. sediminis H-1^T (81.6 and 21.4 %) and R. tibetensis 1351^T (78.5 and 22.9 %). Based on these phylogenetic, chemotaxonomic and genotypic results, strain R-22-1 c-1^T represents a novel species in the genus Rufibacter, for which the name Rufibacter latericius sp. nov. is proposed. The type strain is R-22-1 c-1^T (=CGMCC 1.13570^T=KCTC 62781^T).