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Updated: Dec 6, 2025
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
Published on: March 27, 2017
Pre-Analytical Sampling and Storage Conditions of Amyloid-β Peptides in Venous and Capillary Blood
Marlena Walter1, Jens Wiltfang1,2,3, Jonathan Vogelgsang1,4,5
1University Medical Center Goettingen (UMG), Georg-August-University, Department of Psychiatry and Psychotherapy, Goettingen, Germany.
Abstract:
Previous studies on blood-based biomarkers for Alzheimer's disease suggest a less invasive blood test might be a valuable screening tool for Alzheimer-specific pathology. Pre-analytical sample storage conditions seem to play an important role on amyloid-β (Aβ) stability, impacting reliability and reproducibility. This study shows that Aβ40, Aβ42, and Aβ42/40 levels significantly and early decrease during storage at room temperature in whole blood or plasma. Storing blood samples at 4°C leads to stable Aβ peptide concentrations up to 72 h. In addition, Aβ peptides can be measured in capillary blood with a stable Aβ42/40 ratio up to 72 h at 4°C.
