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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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High-throughput screening for high-efficiency small-molecule biosynthesis.

Matthew Rienzo1, Shaina J Jackson1, Lawrence K Chao1

  • 1Amyris, Inc. 5885 Hollis St. Suite 100 Emeryville, CA, 94608, United States.

Metabolic Engineering
|October 5, 2020
PubMed
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This summary is machine-generated.

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Correction: Mukavi et al. Antiprotozoal Aminosteroid Alkaloids from <i>Buxus obtusifolia</i> (Mildbr.) Hutch. <i>Molecules</i> 2025, <i>30</i>, 4558.

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Developing high-throughput screening methods is crucial for metabolic engineering. This review explores small-scale fermentation and analysis techniques to accelerate the discovery of microbial strains for industrial applications.

Area of Science:

  • Metabolic Engineering
  • Biotechnology
  • Synthetic Biology

Background:

  • Rewiring microbial metabolism is key for cost-effective production of high-value molecules.
  • Accurate modeling of complex cellular systems remains a challenge, necessitating empirical strain development.
  • Systematic creation and evaluation of engineered organism variants are essential for identifying manufacturing-ready strains.

Purpose of the Study:

  • To review strategies for designing high-throughput, small-scale fermentation models.
  • To predict and improve strain performance at large commercial scale.
  • To present established and promising approaches for cell culture and analysis in strain screening.

Main Methods:

  • Focus on microplate- and microfluidics-based screening systems.
Keywords:
Analytical techniquesAutomationBioprocess developmentBioreactorsFermentationHigh-throughput screeningMetabolomicsMicrofluidics

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  • Execution of carefully controlled, parallel, miniature fermentations.
  • High-precision analysis of complex mixtures from fermentation.

    • Main Results:

    • Discussion of strategies for optimizing scalable manufacturing potential.
    • Presentation of approaches from industrial and academic groups.
    • Highlighting the importance of miniature fermentation and advanced analytical techniques.

    Conclusions:

    • High-throughput screening is vital for advancing metabolic engineering.
    • Small-scale fermentation models can predict large-scale performance.
    • Microplate and microfluidics technologies are central to efficient strain development.