Preparation of Size-controlled Giant Vesicles Under Physiological Conditions

Giant vesicles (GVs) are model cell membranes that function as tools for the study of cell membrane properties. Recently, researchers have been calling for GVs of specific sizes for use in studies with precise needs. In this paper, we report a method of forming GVs of specific sizes by using an agarose-swelling approach. The resulting GVs had a narrow size distribution and were successfully formed under physiological conditions.