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Updated: Dec 6, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
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3D Printed Microneedle Array for Electroporation.

Khalil Moussi, Mincho Kavaldzhiev, Jose E Perez

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 6, 2020
    PubMed
    Summary
    This summary is machine-generated.

    3D printed microneedle electrodes enable efficient, low-voltage cell electroporation. This accessible method enhances cell membrane permeability for various in-vitro cell studies and applications.

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    Area of Science:

    • Biotechnology
    • Cell Biology
    • Medical Devices

    Background:

    • In-vitro cell transfection via electroporation is crucial in biology and medicine.
    • Electroporation efficiency depends on cell culture electrical resistance and electrode design.
    • Current microneedle electrode fabrication methods offer limited design flexibility.

    Purpose of the Study:

    • To develop a flexible method for fabricating microneedle electrodes using 3D printing.
    • To investigate the efficacy of 3D printed microneedles for cell electroporation.
    • To enable customized electrical field manipulation for enhanced cell membrane permeability.

    Main Methods:

    • Fabrication of pyramidal-shaped microneedle electrodes using 3D printing technology.
    • Testing of the 3D printed microneedles on HCT116 cancer cells for in-vitro electroporation.
    • Customization of microneedle tip geometry to tailor the electrical field.

    Main Results:

    • Successful fabrication of 3D printed microneedle electrodes with design flexibility.
    • Demonstration of low-voltage (2 V) electroporation, reducing the need for chemical buffers.
    • Effective enhancement of cell membrane permeability in HCT116 cells.

    Conclusions:

    • 3D printing offers a versatile approach for fabricating customized microneedle electrodes.
    • The developed method provides an accessible and efficient platform for in-vitro electroporation.
    • This technology holds potential for diverse cell studies and applications, including multi-culture environments.