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Updated: Aug 12, 2026
16:01
Immunoblot Analysis
Published on: June 20, 2008
An improved immunoblotting procedure for the detection of antibodies against HIV
Journal of Virological Methods
|May 1, 1987
Abstract:
Immunoblotting ('Western blotting') is routinely used for detection of antibodies against HIV in the diagnosis of HIV infection. We describe an improved procedure, which does not require virus purification and is easy to control for 'false-positive' results. The technique also does not produce erroneous results due to reactivity of the developing system with residual cellular proteins or viral antigens and does not give high nonspecific background staining. The technique can be applied to the detection of antibodies to HIV in serum, plasma, and blood products.
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01:20Immunoprecipitation
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01:15Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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