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Related Experiment Video

Updated: Dec 6, 2025

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iQuant Auto: Automated Rapid Test Platform for Immunodiagnostics.

Ramdayalan Kumarasami, Jayaraman Kiruthi Vasan, Jayaraj Joseph

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |October 6, 2020
    PubMed
    Summary

    The iQuant Auto system automates sample preparation for lateral flow immunoassays (LFIA), improving diagnostic accuracy. This automated immunodiagnostic device enhances medical outcomes through rapid, reliable point-of-care testing.

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    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Clinical Diagnostics

    Background:

    • Point-of-care diagnostic devices are crucial for early detection of immunological markers, improving patient outcomes.
    • Lateral flow immunoassays (LFIA) offer rapid, on-site qualitative and semi-quantitative results but often require manual, error-prone sample preparation.
    • Automation is needed to enhance the reliability and efficiency of LFIA sample preparation.

    Purpose of the Study:

    • To introduce and evaluate the iQuant Auto, a fully automated system for sample preparation and analysis in immunodiagnostics.
    • To assess the performance and usability of the iQuant Auto system, particularly in resource-limited settings.
    • To demonstrate the adaptability of the iQuant Auto system for various diagnostic markers using a standardized hardware platform.

    Main Methods:

    • The iQuant Auto system utilizes a pneumatic system for fluid handling and sample preparation, coupled with an image-based fluorescence reader.
    • It operates with test-specific milli-fluidic iQPrep Kits featuring integrated reagent storage and valves.
    • System validation involved testing intra-instrument variation, positional accuracy, and fluid handling capabilities using standard reference cartridges.

    Main Results:

    • The intra-instrument coefficient of variation (CoV) for the mobile kit reader was below 0.62%.
    • Positional accuracy of the system demonstrated a CoV of 0.16%.
    • Average fluid loss was measured at 10 µl per test due to liquid adherence in fluid channels and valves.

    Conclusions:

    • The iQuant Auto offers a fully automated solution for immunodiagnostic sample preparation and analysis, overcoming limitations of manual LFIA methods.
    • The system is designed for ease of operation with a minimalist interface, suitable for less skilled personnel in resource-limited environments.
    • The iQPrep Kit's modular design allows for cost-effective, scalable manufacturing and adaptation to various diagnostic markers, making the iQuant Auto a versatile point-of-care diagnostic tool.