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Updated: Dec 6, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Diagnostic performance of circulating MicroRNAs in acute ischemic stroke: A protocol for systematic review and
Wenzhai Cao1,2, Ting Zhang3, Lizhen Wang4
1Laboratory of Cancer Biology, Key Lab of Biotherapy in Zhejiang, Sir Run Run Shaw Hospital, Medical School of Zhejiang University, Hangzhou, Zhejiang.
Introduction:
Increasing evidences showed differential expression of circulating microRNAs (miRNAs) in patients with acute ischemic stroke (AIS), indicating that miRNAs might serve as promising biomakers in the diagnosis of AIS. However, their accuracy has not been systematically evaluated, so it is necessary to conducted a meta-analysis to evaluate the diagnostic value of miRNAs in AIS patients.
Methods:
PubMed, EMBASE, Cochrane Library, Web of Science, Medline, China National Knowledge Infrastructure (CNKI) will be searched for the relevant studies that explored the potential diagnostic values of miRNAs in AIS patients from inception to August 2020. Data will be extracted by two researchers independently; risk of bias of the meta-analysis will be evaluated by the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2). Data will be synthesised and heterogeneity will be evaluated. All of the above statistical analysis will be performed using Stata V.15.0 and Meta-disc V.1.4.
Results:
This study will assess the pooled diagnostic performance of circulating miRNAs in AIS.
Conclusion:
This study will clarify confusions about the specificity and sensitivity of circulating miRNAs in diagnosing AIS, which could further guide the promotion and application of them.Open Science Framework (OSF) registration number: 2020, August 19. https://osf.io/6tjf3.
Insights
This meta-analysis evaluates circulating microRNAs (miRNAs) as diagnostic biomarkers for acute ischemic stroke (AIS). The study aims to systematically assess their accuracy, sensitivity, and specificity in diagnosing AIS.
Area of Science:
- Biomarkers
- Stroke Research
- Molecular Diagnostics
Background:
- Circulating microRNAs (miRNAs) show differential expression in acute ischemic stroke (AIS) patients.
- miRNAs are potential biomarkers for AIS diagnosis.
- Systematic evaluation of miRNA diagnostic accuracy in AIS is needed.
Purpose of the Study:
- To conduct a meta-analysis evaluating the diagnostic value of circulating miRNAs in AIS patients.
- To systematically assess the accuracy, sensitivity, and specificity of miRNAs for AIS diagnosis.
Main Methods:
- Comprehensive literature search of multiple databases (PubMed, EMBASE, etc.) up to August 2020.
- Independent data extraction and risk of bias assessment using QUADAS-2.
- Statistical synthesis and heterogeneity evaluation using Stata V.15.0 and Meta-disc V.1.4.
Main Results:
- The study will assess the pooled diagnostic performance of circulating miRNAs in AIS.
- This meta-analysis will provide a comprehensive evaluation of diagnostic accuracy.
Conclusions:
- This study will clarify diagnostic performance of circulating miRNAs in AIS.
- Findings will guide the clinical promotion and application of miRNAs as AIS biomarkers.
