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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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In vivo clonal analysis of aging hematopoietic stem cells

Ryo Yamamoto1, Hiromitsu Nakauchi2

  • 1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Lorry I. Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA, 94305, USA; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.

Mechanisms of Ageing and Development
|October 6, 2020
PubMed

Hematopoietic stem cells (HSCs) are characterized by two key features: Self-renewal ability and multilineage differentiation potential (multipotentiality). With aging, these key features gradually change. This is thought to be related to hematological diseases. However, clonal in vivo analysis assessing the potential of HSCs to differentiate along erythroid and platelet lineages ("five-lineage tracing") has not been performed in the aged bone marrow. By contrast, in young HSCs clonal in vivo analysis combined with five-lineage tracing has provided us with novel insights into HSC biology. Understanding HSC aging at the clonal level will help us to elucidate aging mechanisms and disease progression. We review recent progress towards understanding HSC aging at the clonal cell level in the transplantation setting.

Keywords:
Five-lineage tracingHematopoietic stem cell agingLatent hematopoietic stem cellMyeloid-restricted repopulating progenitorMyeloid-restricted stem cell

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