Nitidine chloride possesses anticancer property in lung cancer cells through activating Hippo signaling pathway

Area of Science:

• Oncology
• Molecular Biology
• Cell Biology

Background:

• Nitidine chloride (NC) is recognized for its anti-tumor properties.
• The precise molecular mechanisms underlying NC's anti-cancer effects require further elucidation, particularly in non-small cell lung cancer (NSCLC).

Purpose of the Study:

• To investigate the anti-tumor functions of NC in NSCLC cells.
• To determine the molecular mechanisms and signaling pathways involved in NC-mediated anti-cancer activity.

Main Methods:

• Cell proliferation, migration, and invasion were assessed using CCK-8, colony formation, and Transwell assays.
• Apoptosis, cell cycle, and reactive oxygen species (ROS) levels were analyzed via flow cytometry.
• Protein expression levels of key signaling molecules were measured by western blot.

Main Results:

• NC significantly inhibited NSCLC cell proliferation, migration, and invasion.
• NC treatment induced apoptosis and cell cycle arrest in NSCLC cells.
• NC increased ROS levels and modulated the Hippo signaling pathway by suppressing Lats1, Mob1, and YAP, while enhancing p-Lats1, p-Mob1, and p-YAP1 (ser127) expression.

Conclusions:

• Nitidine chloride demonstrates potent anti-cancer activity against NSCLC cells.
• NC exerts its effects by activating and modulating the Hippo signaling pathway, making it a potential therapeutic agent for NSCLC.