Predicting miRNA-lncRNA-mRNA network in ultraviolet A-induced human skin photoaging
Yao Lin1, Mengbi Lin1, Yufang Liu1
1Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Background/Objective:
Recent researches had reported that microRNAs (miRNAs) played a role in skin photoaging. Our previous study found that long noncoding RNA (lncRNA) expression was changed in the UVA-irradiated skin fibroblasts, but the regulating network of noncoding RNA in UV-induced skin changes has not been elucidated well. Here, we investigated the interactions of miRNA-lncRNA-mRNAs in skin photoaging mechanisms.
Methods:
Human dermal fibroblasts (HDFs) were irradiated with UVA at 10 J/cm2 once a day lasting for 14 days. miRNA expression profiles were detected by high-throughput sequencing. miRNAs changed significantly were identified by qRT-PCR. Functional annotation analysis and pathway enrichment were carried out using Gene Ontology and KEGG, and predicted miRNA-lncRNA-mRNA interactions were performed via bioinformatic analysis.
Results:
34 differentially expressed miRNAs (>1.5-fold changes, P < .05) after UVA irradiation were identified to interact with distinct lncRNAs. miRNA-lncRNA-mRNA network prediction and regulatory role analysis showed that the gene expression of cellular process, cell part, and binding was mainly coordinated in UVA-irradiated fibroblasts. miRNA-lncRNA-mRNA-signal transduction pathway analysis showed that TNF signaling pathway, thyroid hormone signaling pathway, and lysosome were mainly affected after UVA irradiation.
Conclusion:
miRNA-lncRNA-mRNA network played a critical part in skin photoaging. Our research provided novel insights into the repeated UVA-induced skin damage in noncoding RNA regulatory field and might help to further understand the delicate interplay of gene regulation at the noncoding RNA level in photoaged skin and UV-induced skin cancers in future researching and provide novel insights into the repeated UVA-damaging pathology and potential targets for preventing human skin photoaging.
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